Fig. 5

c-Myc is a direct target of miR-1294 in PDAC cells. A. The miRDB, miRWalk, TargetScan, and miRTarBase databases were utilized to predict the target genes of miR-1294. B and C. PANC-1 and MiaPaCa-2 cells were transfected with the miR-1294 mimic, miR-1294 inhibitor or the corresponding negative control, and the expression of c-Myc and SURF4 was evaluated by qRT-PCR. D. Schematic showing the c-Myc-WT and c-Myc-Mut luciferase reporter plasmids. E and F. Relative luciferase activity in PANC-1 and MiaPaCa-2 cells after co-transfection with c-Myc-WT or c-Myc-Mut and the miR-1294 mimic, inhibitor or corresponding negative control. G and H. Western blot analysis was performed to assess the protein levels of c-Myc, E-cadherin, N-cadherin, Vimentin, Snail, Bax, and cleaved caspase-3 after knocking down or overexpressing miR-1294 or circEYA3 in PANC-1 and MiaPaCa-2 cells, as indicated. β-actin was used as the loading control. I. The correlation between the miR-1294 and c-Myc expression levels in 104 paired PDAC patients was analysed by qRT-PCR and Pearson correlation analysis. *P < 0.05, **P < 0.01, ***P < 0.001; ns indicates no significance