Fig. 1

Effect of ADAR1 knockdown on differential gene expression. A Volcano plot representing the RNA-seq differential expression analysis of Cal62 cells comparing siControl versus siADAR1 #1 and #2. ADAR1 is highlighted in the figure among the downregulated genes. B Functional annotation for the down- and up-regulated genes. C RT-qPCR validation of dysregulated genes 72 h after ADAR1 knockdown in Cal62 cells (n = 4). Error bars indicate standard deviations. Asterisks denote statistical significance compared with siControl treatment as assessed with Student’s t-test (two-tailed). * p < 0.05, ** p < 0.01, *** p < 0.001