Fig. 7

Edited and wild-type CDK13 show differences in nuclear speckle and nucleolus localization. Stably-transduced CDK13-WT, CDK13-Edit or Empty vector Cal62 and TPC1 cell lines were used. A and B Confocal images of immunofluorescence staining of the cells with antibodies against HA (green), the nuclear speckle marker SC35 (red) (A) or the nucleolus marker Fibrillarin (Pink) (B). Nuclei were stained with DAPI (blue). Colocalization was analyzed using Fiji-Coloc2 plugin, and the mean of the Pearson R value from 3 independent experiments is represented in the bottom panels. C Upper panels: Representative image of CDK13-Edit Cal62 and TPC1 cells with nucleolar accumulation of CDK13. The images represent the immunofluorescence staining of cells with antibodies against HA (green), SC35 (red) or Fibrillarin (pink). Bottom panel: Graphs show quantification of the percentage of CDK13-WT or CDK13-Edit cells that present with nucleolar accumulation of CDK13 (n = 3 independent experiments). Error bars indicate standard deviations. Asterisks denote statistical significance as assessed with Student’s t-test (two-tailed). * p < 0.05, ** p < 0.01, *** p < 0.001