Fig. 1
The panorama of genetic variation, expression patterns, and therapeutic potential of m6A regulators in small cell lung cancer. a Summary of the current knowledge about the dynamic reversible process of m6A modification in cancer progression; b The mutation frequency of 30 m6A regulators in 110 patients with small cell lung cancer from the International Cohort. Each column corresponds to an individual case. The TMB is displayed as the upper bar plot. The right panel shows the mutation frequency and proportion of each variant type for each regulator. The stacked bar plot on the bottom displays the fraction of conversions in each patient. c The copy number variation frequency of 30 m6A regulators in 53 SCLC cell lines from CCLE. Blue dot, the deletion frequency; Red dot, The amplification frequency. d The location of CNV alteration of the m6A regulators on 23 chromosomes using data from CCLE. e Principal component analysis for the expression profiles of 30 m6A regulators to distinguish small cell lung cancer samples from normal lung samples in GSE40275 cohort. There is no intersection between the two subgroups, indicating the small cell lung cancer samples and normal lung samples were well distinguished based on the expression profiles of m6A regulators. SCLC samples were marked with red and normal lung samples were marked with blue. f The expression detail of 30 m6A regulators between normal lung tissues and small cell lung cancer tissues from GSE40275 cohort. Bule box, normal lung samples; red box, small cell lung cancer samples. g Image flow cytometry shows the expression of HNRNPC and RBMX in control and knockdown cells (NCIH446 and NCIH196). h Transwell migration assays of the migration ability of small cell lung cancer cells (NCIH446 and NCIH196) in the control or knockdown groups. *, **, and *** represent P < 0.05, P < 0.01, and P < 0.001, respectively