Fig. 3
From: Circular RNA circRNF13 inhibits proliferation and metastasis of nasopharyngeal carcinoma via SUMO2
CircRNF13 inhibits NPC growth and metastasis in vivo. A Images of subcutaneous tumor formation in nude mice at 4 weeks. Mice were injected with 2 × 106 CNE2 cells, with knockdown or overexpression of circRNF13. B, C Tumor volumes (B) and tumor weights (C) were measured for each group (n = 7 per group). Data have been represented as mean ± SD. *, p < 0.05; ***, p < 0.001. D Representative images of in situ hybridization for circRNF13 and immunohistochemical staining for Ki-67 expression in subcutaneous tumors (200 × , scale bar: 50 μm). E Images of visible nodules on the lung surface. CNE2 cells transfected with scrambled siRNA, empty vector, sicircRNF13, or circRNF13 overexpression vector were injected into each nude mouse tail vein (n = 7 for each group), and the mice were sacrificed 8 weeks later. F The number of lung metastatic nodules on each lung surface was quantified. Data have been represented as mean ± SD (each point represents one mouse; n = 7 per group; right). ***, p < 0.001. G Representative images of circRNF13 expression, as assessed using in situ hybridization, and lung metastatic tumor foci after H&E staining (200 × , scale bar: 50 μm)