Fig. 5.

rhMG53 promotes G3BP2 nuclear translocation and reduces SG formation in A549 cells sunder ATO-induced stress conditions. A A549 cells were treated with rhMG53 (10 μg/mL), ATO (0.5 mM), or ATO (0.5 mM) plus rhMG53 rhMG53 (10 μg/mL) for 40 min, and the distribution of G3BP2 was analyzed by IHC staining under confocal microscopy. The nucleus was stained with DAPI (blue). Arrows indicate G3BP2 nuclear localization. Quantifications of the (B) cytosolic and (C) nuclear aggregation of G3BP2. *** P<0.005 for the indicated group. Data represents 3 independent experiments. D A549 cells were treated with ATO (0.5 mM) plus rhMG53 rhMG53 (10 μg/mL) (lower panels) for 0, 15min, 60min, and 120 min respectively. The cytoplasmic and nuclear protein extraction were subjected to western blot analysis with anti-G3BP1 and G3BP2 antibodies. GAPDH and Lamin A/C served as loading controls