Fig. 6

UCA1 was a target of miR-182-5p. The relative expression of miR-182-5p was up-regulated by shUCA1(a), and up-regulated by shUCA1 co-transfected miR-182-5p mimics (shUCA1 + miR-182-5p mimics)(b), down-regulated by pcDNA3.1-UCA1(c). Dual-luciferase reporter assays were performed in 786-o or Caki-1 cells co-transfected with UCA1-Wt or UCA1-Mut and miR-182-5p or NC (d). Anti-AGO2 RIP was performed in renal cells transfected with miR-182-5p mimics or NC, followed by RT-qPCR to detect UCA1 (e). ANOVA was used for the comparison of curves of cell proliferation. Cell proliferation was detected in both renal cancer cell lines after co-transfection with shNC+NC, shUCA1 + miR-182-5p inhibitor or shUCA1 + miR-182-5p mimics (f and g). Representative images of EdU assay were shown and the relative fold changes of EdU positive cells were detected after co-transfection with shNC+NC, shUCA1 + miR-182-5p inhibitor or shUCA1 + miR-182-5p mimics (h and i). Assays were performed in triplicate and data were shown as mean ± standard deviation (SD) of those biological replicates or samples (*P < 0.05, **P < 0.01)