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Fig. 4 | Molecular Cancer

Fig. 4

From: circCHST15 is a novel prognostic biomarker that promotes clear cell renal cell carcinoma cell proliferation and metastasis through the miR-125a-5p/EIF4EBP1 axis

Fig. 4

circCHST15 serves as a miR-125a-5p sponge in RCC cells. A Four potential target miRNAs of circCHST15 were predicted by bioinformatic analysis. B Schematic model showed the putative binding sites of eight miRNA candidates associated with circCHST15. C. Relative levels of circCHST15 in A498 and HK2 lysates after RNA pull down using circCHST15 probe or oligo probe. D Relative levels of four miRNAs in A498 and HK2 lysates pulled down by circCHST15 probe or oligo probe. E Relative levels of circCHST15 in A498 and HK2 lysates captured by biotinylated wild-type miRNA-125a-5p (Biotin-miR-125a-5p-wt) or mutant miR-125a-5p(Biotin-miR-125a-5p-mut). F Luciferase reporter assay in HEK293T cells co-transfected with miRNA mimics, psiCHECK-2-wild type circCHST15 (circCHST15-wt) or psiCHECk-2-mutant type circCHST15 (circCHST15-mut) plasmids. G Schematic of circCHST15 wild-type (wt) and mutant (mut) luciferase reporter vectors. H FISH showed the colocalization between circCHST15 and miR-125a-5p in A498 cells. Nuclei were stained with DAPI. CircCHST15 was labeled with Cy3 and miR-125a-5p was labeled with Cy5

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Molecular Cancer

ISSN: 1476-4598