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Fig. 5 | Molecular Cancer

Fig. 5

From: A novel protein AXIN1-295aa encoded by circAXIN1 activates the Wnt/β-catenin signaling pathway to promote gastric cancer progression

Fig. 5

The biological function of circAXIN1. a The RNA and protein levels of circAXIN1 are significantly knocked down by three specific siRNAs, while the expression of linear AXIN1 is unaffected. An illustration of the junction site of circAXIN1 targeted by siRNAs is shown. b Cell proliferation is significantly repressed by circAXIN1 siRNA1 and siRNA3, in both AGS and N87 cells in the EdU assay. c Cell migration of AGS (treated with 10 μg/mL mitomycin for 1 h) and invasion of AGS and N87 cells are both inhibited by circAXIN1 siRNA transfection. d Cell migration is inhibited by circAXIN1 siRNAs in AGS cells (treated with 10 μg/mL mitomycin for 1 h) in the wound healing assay. e Colony formation ability is suppressed in AGS cells with circAXIN1 siRNA transfection. f-h Cell proliferation (f), cell invasion (g), and cell migration (g-h) are enhanced with circAXIN1 or linear AXIN1-295aa transfection in AGS and N87 cells. AGS cells are treated with 10 μg/mL mitomycin for 1 h in a migration assay, 48 h after transfection (g-h). EV, empty vector

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Molecular Cancer

ISSN: 1476-4598