Fig. 5

CircEAF2 functions as a sponge of miR-BART19-3p in EBV + DLBCL. A Gene set enrichment analysis (GSEA) according to circEAF2 expression. B Correlation of miR-BART19-3p expression with circEAF2 expression, APC expression with circEAF2 expression, APC expression with miR-BART19-3p expression in DLBCL tumor samples. Results on tumor samples was further transformed by LogE. C Left panel: Schematic model showed the putative-binding sites for miR-BART19-3p and circEAF2. Middle panel: Luciferase assay was performed in HEK293T cells transfected with the wild-type or mutant APC luciferase reporter and the indicated concentrations of miR-BART19-3p mimics. Right panel: APC-3’UTR luciferase reporter assay was evaluated in HEK293T cells co-transfected with mutant circEAF2 and miR-BART19-3p mimics, or with indicated concentrations of wild-type circEAF2 and miR-BART19-3p mimics. D APC-3’UTR luciferase reporter assay was evaluated in Farage cells co-transfected with wild-type circEAF2 and with miR-BART19-3p mimics. Results were normalized to a Renilla transfection control. E Relative mRNA expression of APC in Farage cells transfected with wild-type circEAF2 and miR-BART19-3p mimics. F Immunoblot assay of APC and nuclear β-catenin proteins in Farage cells transfected with wild-type circEAF2 and miR-BART19-3p mimics. Numbers showed quantification of relative protein amount. GAPDH was used as the control of total protein. Histone3 was used as the endogenous control for nuclear protein. G Schematic model showed the regulator network of circEAF2/miR-BART19-3p/APC/β-catenin in EBV + DLBCL. Created with BioRender.com. GSEA, Gene set enrichment analysis; KEGG, Kyoto Encyclopedia of Genes and Genomes. Data were shown as the mean ± S.D. of three experiments