Fig. 4

PKMYT1 is targeted by miR-485-5p and highly expressed in NSCLC. a Identifying PKMYT1 as the downstream target of miRNA-485-5p using various datasets (Green: StarBase; Pink: Targetscan; Yellow: miRWalk), and the top 50% of the target genes were selected. b The positive correlation between PKMYT1AR and PKMYT1 expressions in TCGA-LAUD dataset. c The negative correlation between miRNA-485-5p and PKMYT1 expressions in TCGA-LAUD dataset. d The prognostic value of PKMYT1 in TCGA-LAUD examined by Kaplan-Meier Plotter. e The relative expression level of PKMYT1 in TCGA-LUAD (Normal: 59; Tumor: 533) and TCGA-LUSC (Normal: 49; Tumor: 502), respectively. f The relative expression of PKMYT1 in GSE30219 datasetset (No-relapse:164; Relapse:114). g The ROC curve for PKMYT1 (AUC=0.954) in LUAD using TCGA dataset. h-i miR-485-5p mimics or inhibitor suppressed or promoted, respectively, PKMYT1 expression in A549 cells tested by Real-time RT-PCR (h) and immunoblot (i). j A schematic graph of the wild-type (WT) and mutant (MUT) PKMYT1 3’-UTR containing luciferase reporter plasmids. k The luciferase activities of the PKMYT1 3’-UTR containing luciferase reporters (WT or MUT) were examined in HEK-293T cells with miR-485-5p mimics or mimic NC co-expression. l-m IHC staining of PKMYT1 proteins in NSCLC cancerous tissues, m is the quantification data for (l). Scale bar=50 μm. n PKMYT1 high expression correlates with worse overall survival using IHC data from tissue microarray (l-m). * P < 0.05, ** P < 0.01, *** P < 0.001