Fig. 2

Altering lncRNA-IUR1 expression regulates Bcr-Abl-transformed cell survival in vitro and tumor growth in vivo. A RT-PCR was performed to examine lncRNA-IUR1 RNA levels in K562 cells stably expressing control (sh-luc) or lncRNA-IUR1 shRNA (sh-IUR1) respectively with or without imatinib treatment. B Cell survival analysis of control and lncRNA-IUR1 knockdown K562 cells upon imatinib treatment (5 μM). Data are presented as mean ± SEM. n = 3, **p < 0.01. C, D Nude mice were subcutaneously injected with control or lncRNA-IUR1 knockdown K562 cells (C). Tumor growth was monitored by bioluminescent imaging, and tumors were excised from the nude mice. Shown were representative images from at least three independent experiments (D). Data are presented as mean ± SEM. n = 3, *p < 0.05. E RT-PCR was performed to examine lncRNA-IUR1 expression in K562 cells stably expressing empty vector (EV) or lncRNA-IUR1 (IUR1) respectively with or without imatinib treatment. F Cell viability of control and lncRNA-IUR1 overexpressing K562 cells, was analyzed in response to imatinib treatment (5 μM). Data are presented as mean ± SEM. n = 3, *p < 0.05. G, H Nude mice were subcutaneously injected with control or lncRNA-IUR1 overexpressing K562 cells. Tumor growth was monitored as described above (G). Shown were representative images from at least three independent experiments (H). Data are presented as mean ± SEM. n = 3, *p < 0.05