Fig. 5

Silencing murine lncRNA-IUR1 in Abl-transformed cells promotes cell survival and the development of leukemia in mice. A-D Scheme of in vivo leukemia transplant (A). Sub-lethally irradiated wild type (WT) C57BL/6 N mice were infused with GFP-positive NS2 cells expressing control (sh-luc) or lncRNA-IUR1 shRNA (sh-mIUR1), or equal volume of PBS. The body weight of indicated mice was monitored for a period of 12 days (B). Quantity of white blood cells (WBCs) (C) and red blood cells (RBCs) (D) in peripheral blood of indicated mice, was detected by blood routine examination. E Bioluminescent imaging analysis of the distribution of GFP-positive control (sh-luc) or lncRNA-IUR1 knockdown (sh-mIUR1) NS2 cells in sub-lethally irradiated C57BL/6 N mice at the 12th day after in vivo leukemia transplantation. PBS group was the negative control. Shown were representative images from at least three independent experiments with similar results. F, G Shown were representative images of spleens from indicated mice at the 12th day after in vivo leukemia transplantation (F). Weight of spleens from indicated mice were measured at the 12th days after in vivo transplantation (G)