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Table 1 list of genes that were knocked out using CRISPR/Cas9 technique in different types of cancer and their effects

From: Strategies to overcome the main challenges of the use of CRISPR/Cas9 as a replacement for cancer therapy

Types of Cancer Target Gene Cell line Animal model Mode of action Delivery Method Function Ref
Breast Cancer P53, PTEN, RB1, NF1 - Mice Knockout lentiviruses For both endocrine and chemotherapy, mutated organoids had a greater response rate for mutated organoids. [47]
Breast cancer miR-23b and miR-27b MCF7 Mice Knockout lentiviruses miR-23b and miR-27b have been shown to be oncogenic miRs, and miR-27b reduces tumor development after knockout. [48]
Breast cancer PTEN, AKT1, PIK3CA NIH3T3 Mice Knockout lentiviruses we show that somatic base editing is feasible and
effective at installing defined missense and nonsense mutations at
endogenous loci in a mouse model of TNBC.
we show that somatic base editing is possible and
effective at installing defined missense and nonsense mutations at
endogenous loci in a mouse model of TNBC.
we show that somatic base editing is feasible and
effective at installing defined missense and nonsense mutations at
endogenous loci in a mouse model of TNBC.
we show that somatic base editing is feasible and
effective at installing defined missense and nonsense mutations at
endogenous loci in a mouse model of TNBC.
we show that somatic base editing is feasible and
effective at installing defined missense and nonsense mutations at
endogenous loci in a mouse model of TNBC.
we show that somatic base editing is feasible and
effective at installing defined missense and nonsense mutations at
endogenous loci in a mouse model of TNBC.
In a TNBC mouse model, somatic base editing may effectively introduce specified missense and nonsense mutations.
[49]
Breast cancer CBEs HEK293-T, MDA-MB-231, MCF-7 - Knockout lentiviruses For ER-driven breast cancer cell growth, unique CTCF-mediated chromatin configurations are required. [50]
Breast cancer AURKA HEK293T, MDA-MB-231, SKBR3, MCF7 - Knockout lentiviruses CHR-6494 might be used in conjunction with MLN8237 to enhance its anti-cancer benefits. [51]
Breast cancer CXCR4 and CXCR7 MDA-MB-231 - Knockout lentiviruses The knockout of CXCR4 and CXCR7 genes reduces the binding ability and activities of CXCL12, slows the growth of TNBC cells, and may be used to treat TNBC. [52]
Breast cancer PARP1 MDA-MB-231,
MDA-MB-436
- Knockout   The effectiveness of PARP1 inhibition with chemotherapy for TNBC treatment varies. [53]
Breast cancer BRCA1 MDA-MB-231, ASC - Knockdown lentiCRISPRv2 vector Breast cancer development is promoted by BRCA1 mutation in the tumor microenvironment. [54]
Breast cancer APOBEC3G MCF10A and HCC1806 - Knockout lipofection multiple clones evaluated for APOBEC3G gene knockout success. [55]
Breast cancer CDK4, SRPK1, DNMT1 MCF10A, HEK 293T and GP2-293 Mice Knockout lentiviruses Transcriptional epistasis influences around 50% of differentially expressed genes in cancer cells. [56]
Breast cancer CDH1 MCF-7 Rats Knockout Plasmid Transfection It is possible to target cancer-related genes using any genome editing technique. [57]
Breast Cancer OPN MDA-MB-231 - Knockout CaCl2 transformation Inactivating osteopontin with CRISPR/Cas9 may overcome radioresistance in breast cancer. [58]
Breast Cancer BRCA MDA-MB-231 - Knockout lentivirus Targeting a group of genes offers new possibilities for PARPi combination treatments. [59]
Breast Cancer TMEM106A MDA-MB-231, MDA-MB-468 - Knockout - In breast cancer, TMEM106A inhibits WDR77 translocation. [60]
Breast and Lung cancer CDK4, p107, TGFβ1 A549 and MCF7 - Knockout - After being challenged with CRISPR cassettes, both cell lines showed a considerable decline in cell count. [61]
Lung cancer PKP2 H1299, A549, H460 - Knockout - Methylation of PKP2 plays an essential factor in radioresistance by stabilizing catenin by CRISPR/Cas9 library screening. [62]
Lung Cancer Trp53, KRas HEK-293T Adult Mice Knockout lentiviruses Using the CRISPR toolset, researchers may rapidly build novel, therapeutically relevant alternative models for biomedical research. [63]
Colon Cancer KRAS HT29, WIDR, HCT116, LS174T, and HEK293T; SW480 and A549; and CFPAC-1 - Knockout two-vector lentivirus system GRB7-PLK1 has a critical axis for RTK tolerance. PLK1 and thus a suitable target for synergizing MEK inhibitors in CRC patients with KRAS mutations. [64]
Colon Cancer Klotho Caco-2 - Knockout - By causing apoptosis, Klotho gene overexpression in Caco-2 cells by CRISPR/Cas9 inhibits cell growth. [65]
Colon cancer uPAR CRL1619, CCL247 - Knockout Okayama-Berg vector Knockout of the uPAR gene Leads to tumor growth inhibition, EGFR downregulation, and an increase in stemness markers. [66]
Prostate cancer Tceal1 Mouse: SP1
Human: PC3M, LNCaP, DU145, CWR22, RWPE
- Knockdown lentivirus TCEAL1 deletion causes a different cell cycle profile than docetaxel alone, with more subG1 cell death and polyploidy. [67]
Prostate cancer miRNA (miR)205, miR221, miR222, miR30c, miR224, miR4553, miR23b, miR505 LNCaP - Knockout Lentivirus Functional classification of prostate cancer-associated miRNAs through CRISPR/Cas9 mediated gene knockout [68]
Prostate cancer BRAF CWR-R1 - Knockout lentiviral MAPK/AR co-targeting may help patients with active MAPK pathways, especially those with oncogenic BRAF mutations. [69]
Prostate cancer TP53 PC-3 - Knock-in lentiviral The impact of CRISPR/Cas9 guided mutant TP53 gene repair in PC-3 human prostate cancer cells [70]
Prostate cancer ECE1, ABCA12, BPY2, EEF1A1, RAD9A, and NIPSNAP1 DU145 and PC3 - Knockdown lentiviral Prostate cancer metformin Resistance related gene screening using CRISPR-Cas9. [71]
Ovarian cancer EGFL6 SKOV3 - Knockout Lentivirus EGFL6 knockout by CRISPR/Cas9 inhibited tumor angiogenesis. [72]
Ovarian cancer ZNF587B and SULF1 A2780, SKOV3, IOSE80 - Knockout Lentivirus Based on genome-scale CRISPR/Cas9 screening, loss of ZNF587B and SULF1 led to cisplatin resistance. [73]
Ovarian cancer AR and Nanog expression A2780, SKOV3 - Knock in Lentivirus Nanog interaction with androgen receptor signaling axis regulates ovarian cancer stem cells using CRISPR/Cas9. [74]
Ovarian cancer ITK SKOV3 Human Knockout Lentivirus For ovarian cancer metastasis, ITK (IL2 Inducible T Cell Kinase) may be a possible cancer suppressor gene. [75]
thyroid cancer AXIN1 ACT-1 - Knockout Viral vector CRISPR/Cas9 has been used to effectively create an ACT-1 undifferentiated thyroid cancer cell line lacking the AXIN1 gene. [76]
Liver Cancer PTPMT1 HCC - Knockout and knockdown lentiCas9-Blast vector CRISPR-Cas9 knockdown library screening revealed PTPMT1 in the production of cardiolipin as critical to survival in hypoxia in liver cancer. [77]
Liver cancer Pten, Rb1, and Ctnnb1 - Mice - px459 V2.0 vector CRISPR/Cas9-induced Liver cancer mouse model: Longitudinal imaging of liver cancer Using MicroCT and nanoparticle contrasting agents. [78]
Liver
Cancer
Traf3 HepG2 - Knockout Lentiviral The CRISPR/Cas9 method improved HepG2 cell proliferation, migration, and invasion and provided a helpful tool for researching Traf3 function and mechanism. [79]
Liver cancer ARID1A, HCC Pig Knockout - CRISPR/Cas9 editing of pig liver cancer cells to create genetically customized cancer cells. [80]