Fig. 1
From: A nuclear function for an oncogenic microRNA as a modulator of snRNA and splicing
CLEAR-CLIP identifies U6 snRNA as the top miR-10b interacting transcript. a Schematics of CLEAR-CLIP procedure, described in details in the Methods. b Top genes represented by the highest number of unique reads identified in chimeric miR-10b libraries in LN229 cells. c Summary of miR-10b chimeras and binding site distribution in mRNAs. More details are provided in Fig. S1, Table S1 and Table S2. d miR-10b binding site within U6 snRNA, at the 3′ end (positions 79 to 102) of U6. e miR-10b-U6 chimera detected by PCR, with one primer corresponding to miR-10b and another to U6, in CLEAR-CLIP libraries produced from glioma LN229 cells, GBM8 stem cells, and non-glioma HEK-293 and SH-Sy-5y cell lines (n = 3)