Fig. 2
From: CircMET promotes tumor proliferation by enhancing CDKN2A mRNA decay and upregulating SMAD3
High-expressed circMET promotes the proliferation of NONO-TFE3 tRCC. (A-F) The effects of circMET knockdown or overexpression on the proliferation of UOK109 and 786-O cells respectively were examined by CCK-8 assay (A-B), colony formation assays (C-D) and tumor sphere formation (E–F). (G-H) EdU assays were used to detect the proliferation rate of UOK109 and 786-O cells after transfected with indicated lentivirus. (I-J) Cell cycle phases were analyzed using flow cytometry after transfected with indicated lentivirus. (K-L) circMET mediates the expression of genes related with cell proliferation and cell cycle in UOK109 and 786-O cells. (M–N) Nude mice were injected subcutaneously with 786-O cells and tumor formation monitored over a period of several weeks. (O) The tumor volume was measured as indicated. (P) Representative H&E staining of xenograft tumors. The data are presented as the mean ± SD, ***P < 0.001