Fig. 6

ASO inhibits CRC cell proliferation, migration and invasion in vitro by controlling circRHOBTB3 circularization and secretion. (A) Schematic of the functional mechanism and chemical modification of ASO. (B) Expression of circRHOBTB3 in ASO-NC- and ASO-cir-treated HCT116, RKO and SW480 cells. (C) Expression of circRHOBTB3 in ASO-NC- and ASO-cir-treated HCT116 cells. (D) Cell proliferation and (E) quantification of migration and invasion assays of ASO-NC- and ASO-cir-treated HCT116, RKO and SW480 cells. (F) Expression of circRHOBTB3 in the cytosome and exosomes of ASO-NC-, ASO-exo1-, and ASO-exo2-treated SW480 cells. (G) Cell proliferation and (H) quantification of migration and invasion assays of ASO-NC- and ASO-exo2-treated SW480 cells. (I) Western blotting and RT-qPCR of the anti-SNF8 RIP assay in ASO-NC- and ASO-exo-treated SW480 cells. (J) Relative circRHOBTB3 or RHOBTB3 expression and (K) cell proliferation and (L, M) quantification of cell migration and invasion assay of ASO-NC, ASO-exo, ASO-cir and ASO-cir combined with ASO-exo treated SW480 (L) Mock and (M) KO cells. All experiments were repeated for three times, data were shown as mean ± SD (B, C, D, F, G,J, K) or mean ± SEM (E, H, I, L, M), * P < 0.05, ** P < 0.01, *** P < 0.001, NS P > 0.05, in Student’s test (B, C, D, F, G,J, K) or paired Student’s test (E, H, I, L, M)