Fig. 3
From: Targeting HNRNPU to overcome cisplatin resistance in bladder cancer
Loss of HNRNPU in T24 cells mediates cisplatin sensitivity in vitro. (A) Cells were transfected with six sgRNAs per gene, and the fold change of sensitivity enhancement (FSE) of the 21 top decreased genes in T24 cells from our screen were determined using HCS analysis. Results were normalized to the control group. FSE was calculated as the cell count fold change of ((NC + drug) /NC) / ((Treatment + drug) / Treatment), NC, negative control (B) sgRNA of four selected genes were transfected into cells, and the FSE of four selected genes, including HNRNPU, CCDC1, PRKCDBP and OCLM, were detected. (C) CRIPSR-based sgRNA editing efficiency of the top 9 sgRNA that targets the selected genes. (D) HNRNPU protein expression levels in T24, RT4, HT1197, SW780, RT112, and HT1376 cells. Simple linear regression of HNRNPU expression levels and cisplatin IC50 values in bladder cancer cells were calculated in GraphPad Prism 7.0 (E) Representative blot of HNRNPU in the cells after different treatments