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Fig. 3 | Molecular Cancer

Fig. 3

From: De novo generation of the NPM-ALK fusion recapitulates the pleiotropic phenotypes of ALK+ ALCL pathogenesis and reveals the ROR2 receptor as target for tumor cells

Fig. 3

Immunophenotype of NA cells. A. CD4+ and CD8+ cell survival overtime (analyzed by flow cytometry) for primary activated T lymphocytes (between 0 and 20 days post-activation) and NA cells (between 0 and 15 days post-transfection). The time of transfection is indicated by a dashed line for primary activated T lymphocytes (n = 3). B. CD30 expression for each cell population (CD4+ or CD8+ cells) for primary activated T lymphocytes (between 0 and 20 days post-activation) and NA cells (between 0 and 15 days post-transfection). The transfection timepoint is indicated by a vertical dashed line for the primary activated T lymphocytes (n = 3). C. Flow cytometry assessment of the CD4, CD8, CD3 and CD30 markers. For all donors, cell populations were obtained at day 15 post-transfection. Subpopulations (CD4 + CD8-, CD8 + CD4- and CD4-CD8-) were also analyzed for Donor 1 (left). D. Representative image of metaphases (FISH analysis) of NA1, NA2 and NA3 cells, 1 month post-transfection. Break-apart probe (green + red): ALK gene; blue probe: NPM1 gene. E. Flow cytometry assessment of CD4, CD8, CD3 and CD30 expression in NA1, NA2, NA3 cells (1 month post-transfection, time point corresponding to mice injections)

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Molecular Cancer

ISSN: 1476-4598