Fig. 3
From: Demethylase ALKBH5 suppresses invasion of gastric cancer via PKMYT1 m6A modification
PKMYT1 was identified as a downstream target of ALKBH5 (A) Filtering process of target genes among meRIP-seq, mRNA-seq, GEPIA database and literature search. (B) MeRIP-qPCR analysis of PKMYT1 after ALKBH5 knockdown in SGC-7901 cell. (C-D) The protein and mRNA level of PKMYT1 in ALKBH5 overexpression GC cell. (E–F) The protein and mRNA level of PKMYT1 in ALKBH5 knockdown GC cells. (G) RNA Pulldown assay of ALKBH5 using NC or PKMYT1 probe. (H) RIP-qPCR assay of PKMYT1 enrichment by ALKBH5 protein. (I-J) Representative IHC pictures of PKMYT1 in GC tissue microarray (TMA) and the comparison of area density in PKMYT1-staining (scale bars = 100 µm). (K) Survival analysis of PKMYT1 expression in GC patients (p = 0.024, log-rank test). (L) MRNA expression comparison of PKMYT1 in TCGA database. (M) Kaplan–Meier OS analysis of PKMYT1 expression in patients with GC (HR = 1.57, p = 9.8e-07, log-rank test). (N) ROC analysis of PKMYT1 expression in TMA. (O) Expression correlation between PKMYT1 and ALKBH5 in TMA. Pearson r = -0.23339, p = 0.0158. (P-R) Migration and invasion ability of PKMYT1 overexpression after LV-ALKBH5 in HGC-27 cell (scale bar = 200 um). (S-U) Migration and invasion ability of si-PKMYT1 after ALKBH5 knockdown in SGC-7901 cell (scale bar = 200 um)