Fig. 5

CircLHFPL2 acted as ceRNA to sponge miR-556-5p and miR-1322. A Schema of predicted miRNAs sponged by circLHFPL2 by using Circbase (https://starbase.sysu.edu.cn/) and Circinteractome (https://circinteractome.nia.nih.gov/). B Luciferase reporter assay confirmed the interaction between circLHFPL2 and predicted miRNAs. C Fluorescence in situ hybridization (FISH) examined the location of circLHFPL2 with miR-1322 and miR-556-5p. D and G Luciferase reporter assay confirmed the interaction between circLHFPL2 and miR-556-5p (D) or miR-1322 (G). E and H RNA pull down assay confirmed the interaction between circLHFPL2 and miR-556-5p (E) or miR-1322 (H). F and I Correlation between circLHFPL2 and miR-556-5p (F), circLHFPL2 and miR-1322 (I) analyzed by Pearson’s correlation analysis. J Binding of circLHFPL2 to 3′-end biotinylated miR-556-5p (left panel) and 3′-end biotinylated miR-1322 (right panel) in HCT116 cells with circTP63 overexpression. K Relative expression of miR-556-5p (left) and miR-1322 (right) was determined via qRT-PCR of tumor tissues from 30 CRC patients with PIK3CA Mut and 30 patients with PIK3CA WT. L and M qRT-PCR showed that SC-79 upregulated miR-556-5p and miR-1322 expression in HCT116 (L) and DLD1 (M) cells. Data are presented as mean ± SEM; n ≥ 3. *p < 0.05; **p < 0.01