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Fig. 1 | Molecular Cancer

Fig. 1

From: Interaction of lncRNA MIR100HG with hnRNPA2B1 facilitates m6A-dependent stabilization of TCF7L2 mRNA and colorectal cancer progression

Fig. 1

Regulation of EMT in CRC by MIR100HG. a Immunofluorescence of αPKC, GP135, Ezrin and p120-catenin in CC and CC-CR cells in 3D culture. b Immunofluorescence of E-cadherin, ZO-1, N-cadherin and Vimentin in CC and CC-CR cells in 3D culture. c Immunoblots of indicated proteins from 3D-cultured CC and CC-CR cell lysates. β-actin served as a loading control. Representative of three independent experiments. d Correlation analysis of MIR100HG expression and EMT score in CRC patients (n = 2375). e GSEA of an EMT gene signature in CRC samples with high MIR100HG expression versus those with low MIR100HG expression in TCGA database (n = 478). The median expression of MIR100HG was used as the cut-off. f Correlation analyses of expression of MIR100HG and EMT markers in TCGA database (n = 433). g-j qPCR and immunoblots of EMT markers in indicated cells, n = 3 independent biological replicates. **P < 0.01, *P < 0.05. Data represent mean ± s.d

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Molecular Cancer

ISSN: 1476-4598