Fig. 6

USP10 interacts with p53 and suppresses BC cell growth. a Co-IP assays to analyze the direct interaction between USP10 and p53 in MCF-7 cells using anti-USP10 or anti-p53, respectively. b Co-IP was applied in MCF-7 cells transfected with indicated full-length or truncated USP10 plasmids using anti-Flag. c Ubiquitination of p53 was analyzed in MG132-treated MCF-7 cells bearing Flag-Mock and Flag-USP10 or si-NC, si-USP10#1 and si-USP10#2. d Western blot analyses of hypoxic MCF-7 and MDA-MB-453 cells after knockdown of USP10 with indicated antibodies. e and f CCK-8 (e) and colony formation assays (f) were conducted in MCF-7 and MDA-MB-453 cells transfected with indicated siRNAs under hypoxia. g and h Cell cycle progression (g) and apoptosis rate (h) of hypoxic MCF-7 and MDA-MB-453 cells were analyzed by flow cytometry after enforced expression of USP10. Data are showed as mean ± SD and representative of three independent experiments in (e–h). *P < 0.05, **P < 0.01, ***P < 0.001