Skip to main content
Fig. 10 | Molecular Cancer

Fig. 10

From: AP4 suppresses DNA damage, chromosomal instability and senescence via inducing MDC1/Mediator of DNA damage Checkpoint 1 and repressing MIR22HG/miR-22-3p

Fig. 10

AP4 confers resistance towards 5-FU via MDC1. A The indicated cells were treated with increasing concentrations of 5-FU for 48 h. Then the IC50 was determined by an MTT assay. B-E The indicated cells were transfected with the indicated oligonucleotides for 48 h and subsequently treated with increasing concentrations of 5-FU for 48 h. Then the IC50 was determined by an MTT assay. F The DLD-1 pRTR-AP4-VSV cell pool was treated with DOX for 48 h and subsequently with increasing concentrations of 5-FU for 48 h. For the last 48 h fresh DOX was added. Then the IC50 was determined by an MTT assay. G-J Colony formation assay of the indicated cells transfected with the indicated oligonucleotides or plasmids for 48 h and then subjected to treatment with 10 μM 5-FU for 48 h and then cultured for additional 3 weeks. K and L Colony formation assay, as described in (G), of DLD-1 pRTR-AP4-VSV pools after DOX treatment for 48 h after transfection of the indicated oligonucleotides for 48 h. In panels G-L, the mean + SD is provided. *:p < 0.05, **:p < 0.01, ***:p < 0.001

Back to article page

Molecular Cancer

ISSN: 1476-4598