Fig. 7

CircSPON2 promotes PCa cell proliferation and migration. A qRT-PCR analysis of circSPON2 and SPON2 mRNA in PC-3 and DU145 cells treated with negative control (NC) or circSPON2 shRNAs. B-D CCK-8, plate colony-formation and EdU incorporation assays were performed to determine the proliferation abilities of PC-3 and DU145 cells treated with negative control (NC) or circSPON2 shRNAs. E Image of subcutaneous tumor xenografts in negative control (NC) or circSPON2 shRNAs groups. F The tumor growth curves of xenografts were plotted in negative control (NC) or circSPON2 shRNAs groups. G The tumor weights of xenografts were evaluated. H Representative IHC staining micrographs of Ki-67 in tumor xenografts were conducted. Scale bar = 20 μm. I and J Wound healing and transwell assays were performed to determine the migratory capabilities of PC-3 and DU145 cells treated with negative control (NC) or circSPON2 shRNAs. K BALB/c nude mice injected with cells (NC and shRNA-1; five mice per group) via tail vein were imaged at day 40 by in vivo imaging system to evaluate the whole body metastasis. L Representative images of H&E staining of lung metastasis loci. Data were showed as mean ± SD. **P < 0.01