Fig. 8

CircSPON2 serves as a sponge of tumor suppressor miR-331-3p in PCa. A Seven miRNAs were predicted as the potential targets of circSPON2 by CRI and ENCORI databases. B CircSPON2 in PC-3 and DU145 cells was pulled down by a circSPON2-specific probe and determined by qRT-PCR assays. C Relative levels of candidate miRNAs were determined by qRT-PCR assays after being pulled down by circSPON2-specific probe or control oligo probe. D Relative levels of miR-331-3p were detected by qRT-PCR assays in PC-3 and DU145 cells treated with vector or oe-circSPON2. E Relative levels of miR-331-3p were detected by qRT-PCR assays in PC-3 and DU145 cells treated with negative control (NC) or circSPON2 shRNAs. F Luciferase reporter assay of PC-3 and DU145 cells co-transfected with miR-331-3p mimics and luciferase reporter plasmid containing wild-type (WT) and miR-331-3p binding site mutated (Mut) circSPON2. G FISH with Cy3-labeled circSPON2 probes (red) and FITC-labeled miR-331-3p (green) were performed to detect the location of circSPON2 and miR-331-3p in PC-3 cells. H The correlation analysis between the expression levels of miR-331-3p and circSPON2 in our own patient cohort (n = 248; r = -0.5191, P < 0.01). I The correlation analysis between the expression levels of PRMT5 and circSPON2 in our own patient cohort (n = 248; r = 0.4746, P < 0.01). Data were showed as mean ± SD. *P < 0.05, **P < 0.01