Fig. 9

CircSPON2 promotes PCa progression by sponging miR-331-3p. A-C CCK-8, plate colony-formation and EdU incorporation assays were performed to determine the proliferation abilities of PC-3 and DU145 cells treated with control, oe-circSPON2, oe-miR-331-3p or oe-circSPON2 + oe-miR-331-3p. D Image of subcutaneous tumor xenografts in control, oe-circSPON2, oe-miR-331-3p or oe-circSPON2 + oe-miR-331-3p groups. E The tumor growth curves of xenografts were plotted in control, oe-circSPON2, oe-miR-331-3p or oe-circSPON2 + oe-miR-331-3p groups. F The tumor weights of xenografts were evaluated. G Representative IHC staining micrographs of Ki-67 in tumor xenografts were conducted. Scale bar = 20 μm. H and I Wound healing and transwell assays were performed to determine the migratory capabilities of PC-3 and DU145 cells treated with control, oe-circSPON2, oe-miR-331-3p or oe-circSPON2 + oe-miR-331-3p. J BALB/c nude mice injected with cells (control, oe-circSPON2, oe-miR-331-3p or oe-circSPON2 + oe-miR-331-3p; Five mice per group) via tail vein were imaged at 40 days by in vivo imaging system to evaluate the whole metastasis. K Representative images of H&E staining of lung metastasis loci. Data were showed as mean ± SD. **P < 0.01