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Fig. 3 | Molecular Cancer

Fig. 3

From: Circular RNA circPOLR2A promotes clear cell renal cell carcinoma progression by facilitating the UBE3C-induced ubiquitination of PEBP1 and, thereby, activating the ERK signaling pathway

Fig. 3

CircPOLR2A promoted malignant biological properties in cRCC cells. a, b The expression of circPOLR2A and POLR2A mRNA was detected after circPOLR2A knockdown (a) or overexpression (b) in cRCC cells. c, d CCK-8 assay indicated the growth rate of cRCC cells transfected with lentivirus for circPOLR2A knockdown (c) or overexpression (d). Two-way ANOVA was used to evaluate significant differences. e Colony formation assay for cRCC cells. f EdU incorporation assay was performed to assess DNA synthesis in cRCC cells. Red fluorescence represented EdU-positive cells, while blue fluorescence represented total cells. Scale bar, 100 μm. g Cell migration and invasion assays of cRCC cells treated with circPOLR2A knockdown or overexpression. Left, transwell assay, scale bar, 40 μm; right, wound healing assay, scale bar, 100 μm. h Statistical analysis of the cell migration and invasion assays. i, j The effect of circPOLR2A knockdown or overexpression on cell apoptosis via flow cytometry assay

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Molecular Cancer

ISSN: 1476-4598