Fig. 1
From: CircBCAR3 accelerates esophageal cancer tumorigenesis and metastasis via sponging miR-27a-3p
CircBCAR3 exhibits upregulation in esophageal cancer. A Differentially expressed circRNAs in esophageal cancer based on both GSE150476 and GSE112496 datasets. B Expression of hsa_circRNA_103225, hsa_circRNA_404013, hsa_circRNA_102471, hsa_circRNA_007624, and hsa_circRNA_082546 in hypoxia EC109 cells was revealed by PCR. C PCR showed the significant higher expression of circBCAR3 in 45 esophageal carcinoma tissues than adjacent tissues. Student t-test was conducted. D PCR showed the significant higher expression of circBCAR3 in esophageal carcinoma cell lines than normal Het-1A cells. One-way ANOVA was conducted. E CircBCAR3 is derived from the 2–5 exons of BCAR3 pre-mRNA. F PCR analysis of BCAR3 mRNA and circBCAR3 expression in RNase R-treated EC109 and KYSE150 cells. Student t-test was conducted. G PCR analysis of BCAR3 mRNA and circBCAR3 expression in actinomycin D-treated EC109 and KYSE150 cells. Two-way ANOVA was conducted. H PCR indicated the backsplicing of circBCAR3. CircBCAR3 was amplified by divergent primers in cDNA but not in gDNA with GAPDH as a negative control. I FISH revealed the subcellular location and expression of circBCAR3 in EC109 and KYSE150 cells. ** p < 0.01, *** p < 0.001