Fig. 4
From: CircBCAR3 accelerates esophageal cancer tumorigenesis and metastasis via sponging miR-27a-3p
Knockdown of circBCAR3 reversed the effects of hypoxia on esophageal cancer cells in vitro. A CCK-8, B colony formation and C EdU assays were performed to reveal the viability and proliferation of EC109 and KYSE150 cells after hypoxia treatment or cotreatment of hypoxia+sh-circBCAR3. D Wound healing and E transwell assays were used to assess the migration and invasion of esophageal cancer Cells. F Fe2+ levels and G lipid ROS levels were assessed. H Cell morphological changes were observed using transmission electron microscopy to confirm ferroptosis. I GPX4 protein was assessed by western blotting. * p < 0.05, ** p < 0.01 vs control, #p < 0.05, ##p < 0.01 vs hypoxia. Data for CCK-8 assay were analyzed using two-way ANOVA and data for the remaining assays were analyzed using one-way ANOVA