Fig. 6
From: CircBCAR3 accelerates esophageal cancer tumorigenesis and metastasis via sponging miR-27a-3p
CircBCAR3 acts as the sponge of miR-27a-3p in esophageal cancer cells. A Binding sequences of miR-27a-3p on circBCAR3. The complementary bases are linked in vertical line. B Luciferase reporter assay was performed for revealing the binding between miR-27a-3p and circBCAR3. C, D RIP assay was conducted for detecting the binding of circBCAR3 and miR-27a-3p to the Ago antibody. E PCR tested miR-27a-3p expression in EC109 and KYSE150 cells with circBCAR3 knockdown or overexpression. F FISH assay revealed the expression and subcellular location of miR-27a-3p and circBCAR3 in EC109 and KYSE150 cells. ** p < 0.01, *** p < 0.001