Fig. 10

CircGPR137B inhibits in vivo lung metastasis of HCC. A Ex vivo imaging of the luciferase signals in orthotopic liver tumors with peritoneal infiltration by stable transfection with circGPR137B or control group into luciferase-labeled HepG2 cells. B Schematic representation of the comparison of orthotopic liver tumors, and statistical analysis of the total liver weight between circGPR137B and control groups. C Schematic representation of the comparison of metastatic pulmonary tumors, and statistical analysis of the total lung weight between circGPR137B and control groups. D HE staining of the tumor tissue cells from orthotopic liver tumors and metastatic pulmonary tumors in circGPR137B and control groups and IHC analysis of the Ki-67 proliferation index and FTO expression levels in orthotopic liver tumors and metastatic pulmonary tumors between circGPR137B and control groups. E. RT-qPCR analysis of the expression levels of circGPR137B, miR-4739 and FTO in orthotopic liver tumors between circGPR137B and control groups