Fig. 5

CircGPR137B acts as a sponge for miR-4739 in HCC. A Schematic representation of the binding sites between miR-4739 and WT circGPR137B 3’UTR. B RT-qPCR analysis of the effects of circGPR137B overexpression on miR-4739 expression in HepG2 and Hep3B cells. C Comparison of the luciferase activity of circGPR137B 3’UTR after treatment with miR-4739 mimics in HepG2 and Hep3B cells. D. RIP analysis of the enrichment levels of circGPR137B and miR-4739 pulled down from Ago2 or IgG protein in HepG2 and Hep3B cells. E FISH analysis of the co-localization of circGPR137B with miR-4739 in HepG2 cells. Blue color: DAPI, Red color: miR-4739, Green color: circGPR137B. MTT (F) and Transwell analysis (G) of the cell proliferation and invasion after transfection with circGPR137B lentiviruses and (or) miR-4739 mimics in HepG2 and Hep3B cells. Data are the means ± SEM of three experiments. *P < 0.05, **P < 0.01