Fig. 7

circFIRRE promotes OS tumorigenesis and angiogenesis by miR-486-3p/miR-1225-5p-LUZP1 axis in vitro. A Schematic illustration exhibited overlapping of the target genes of miR-486-3p and miR-1225-5p predicted by three algorithms and RNA-sequencing data. B The relative expression of LUZP1 declined in 35 paired OS samples relative to adjacent normal controls through RT-qPCR detection, respectively. C-E Pearson correlation analysis illustrated that LUZP1 was positively correlated with circFIRRE, while negatively correlated with miR-486-3p and miR-1225-5p in 35 paired samples. F Dual luciferase assay was performed to verify whether miR-486-3p and miR-1225-5p could bind to predicted binding sites of LUZP1 (n=3 in each group). G-H Relative mRNA and protein expression of LUZP1 were suppressed under circFIRRE silencing, while the downregulation of LUZP1 was retarded by miR-486-3p and (or) miR-1225-5p inhibitors. I-L Transwell assay and tube formation assay were conducted in MG63, U2OS and HUVEC cells on the third day after co-transfection. Scar bars=400 μm and 1mm. Values are presented as mean ± SD; the bar charts, error bars and dots represent the quantitative analysis of 3 independent experiments; (B, G, 2-tailed Student t test; F, J, L, two-way ANOVA); *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001; ns = not significant