Fig. 1

T1: The expression and clinical significance of TIPE3 in PC. A Representative IHC staining of TIPE3. B IHC sum scores were applied to assess TIPE3 in PC specimens. C) IHC staining of TIPE3 in PC tissues with or without lymph node metastasis. D IHC sum scores were applied to assess TIPE3 in PC specimens with or without lymph node metastasis. E TIPE3 expression in negative lymph node and metastatic lymph node. F Survival analysis according to TIPE3 expression in 188 PC patients. G Prognostic Nomogram of 188 PC patients. H Calibration curves of the OS nomogram. (I-K) DCA for the OS nomogram at 1-year I 2-year J and 3-year K. ***, P < 0.001. T2. The clinical significance of TIPE3 A IHC sum scores were applied to determine TIPE3 in PC. B IHC sum scores were applied to assess TIPE3 expression in PC specimens. C Survival analysis using TIPE3 in 66 PC patients. *, P < 0.05; ***, P < 0.001. T3. TIPE3 promoted malignant biological behaviors of PC cells. A Detection of TIPE3 mRNA expression using qRT-PCR. B TIPE3 expression were detected after TIPE3 silencing or overexpression using qRT-PCR. C CCK8 analysis was conducted after TIPE3 silencing in AsPC-1 and PANC-1 cells. D CCK8 analysis was conducted after TIPE3 overexpression in PC cells. E Trans-well assays were performed after TIPE3 silencing or overexpression in AsPC-1 cells. F Trans-well assays were performed after TIPE3 silencing or overexpression in PANC-1 cells. *, P < 0.05; **, P < 0.01; ***, P < 0.001. T4. TIPE3 promoted tumor progression and metastasis in mice. A Tumor growth curve of orthotopic xenograft mouse. B Representative pictures of primary tumors in pancreas. B Tumor volume in NC, shTIPE3 and TIPE3 group, respectively. D Tumor weight in NC, shTIPE3 and TIPE3 group, respectively. E Survival curves of different groups. F The representative of bioluminescent images in metastatic mouse model. G Number of liver metastatic foci were recorded. H Number of peritoneal metastatic tumors were recorded. *, P < 0.05; **, P < 0.01; ***, P < 0.001