Fig. 2

T1: TIPE3 increased RAC1 in PC. A IHC staining of RAC1. B IHC sum scores were applied to determine RAC1 expression. C IHC sum scores of RAC1 in PC tissues with low or high TIPE3 expression. D Western-blot was conducted to detected RAC1 expression after TIPE3 silencing or overexpression. E Quantified results of western-blot for RAC1 expression in PC cells. F IHC staining of TIPE3, RAC1, RhoA and MMP9 in orthotopic xenograft tumors. G IHC sum scores of TIPE3, RAC1, RhoA and MMP9 expression in orthotopic xenograft tumors. *, P < 0.05; **, P < 0.01; ***, P < 0.001. T2. TIPE3 accelerated the malignant behaviors of PC cells in a RAC1-dependent manner. A NSC23766 (50 μM) was used in CCK8 analysis. B CCK8 assays was measurement after RAC1 silencing in PC cells. C Trans-well assays after treatment of NSC23766 (50 μM). D Trans-well assays were measurement after RAC1 silencing in PC cells. E CCK8 assays were measurement in TIPE3 silencing PC cells that pretreated with NSC23766 (50 μM) or transfection with RAC1 siRNA. F CCK8 assays were conducted in TIPE3 overexpressed PC cells that pretreated with NSC23766 (50 μM) or transfection with RAC1 siRNA. G Trans-well assays were conducted in TIPE3 silenced PC cells that pretreated with NSC23766 (50 μM). H Trans-well assays were conducted in TIPE3 silenced PC cells that transfected with RAC1 siRNA. I Trans-well assays were conducted in TIPE3 overexpressed PC cells that pretreated with NSC23766 (50 μM). J Trans-well migration and invasion assays were conducted in TIPE3 overexpressed PC cells that transfected with RAC1 siRNA