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Fig. 1 | Molecular Cancer

Fig. 1

From: CircSMARCC1 facilitates tumor progression by disrupting the crosstalk between prostate cancer cells and tumor-associated macrophages via miR-1322/CCL20/CCR6 signaling

Fig. 1

circSMARCC1 validated and characterized in PCa cells. A The cluster heat map demonstrated the top 20 circRNAs differentially expressed in four pairs of plasma samples from PCa and BPH patients. B, C Schematic representation of the formation of circSMARCC1 by cyclization of exons 14, 15, and 16 of the SMARCC1 gene. The back splice junction sequence and RT-PCR product of circSMARCC1 were verified by Sanger sequencing and agarose gel electrophoresis, respectively. D The localization of circSMARCC1 observed in PCa tissues (scale bar, 20 μm) and cells (scale bar, 10 μm) detected by FISH. Nuclei were stained with DAPI. E Analysis of the cellular localization of circSMARCC1 by nuclear-cytoplasmic fractionation experiment. GAPDH was used as a control for cytoplasmic proteins and U6 was used as a nuclear control. F qRT-PCR was performed to determine the abundance of circSMARCC1 and SMARCC1 mRNA in PCa cells treated with RNase R at the indicated time points. G RNA expression of circSMARCC1 and SMARCC1 in PCa cells analyzed by qRT-PCR after treatment with actinomycin D for 4 h,8 h,12 h and 24 h.The data are presented as mean ± SD.**p < 0.01, ***p < 0.001, ****p < 0.0001

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Molecular Cancer

ISSN: 1476-4598