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Fig. 3 | Molecular Cancer

Fig. 3

From: CircSMARCC1 facilitates tumor progression by disrupting the crosstalk between prostate cancer cells and tumor-associated macrophages via miR-1322/CCL20/CCR6 signaling

Fig. 3

CircSMARCC1 promotes proliferation, migration and invasion of PCa cells. A three siRNAs (si-#01,02,03) targeting circSMARCC1 were constructed to silence its expression and confirmed by qRT-PCR. B The relative expression of circSMARCC1 and SMARCC1 in PCa cells transfected with circSMARCC1 overexpressing or knockdown lentivirus by qRT-PCR. C-E Assessment of cell proliferation capacity by colony formation, EdU assay (scale bar, 100 μm) and CCK-8 assay. F Western blot analysis evaluated expression of cell cycle-associated proteins and EMT biomarkers following overexpression or knockdown of circSMARCC1. G Flow cytometric analysis of changes in the cell cycle profile of PCa cells stably transfected with circSMARCC1. H, I Transwell assays and wound healing assays assessed the migration and invasion abilities of PCa cells stably transfected with circSMARCC1 (Scalebar, 50 μm). The data are presented as the mean ± SD, **p < 0.01, ***p < 0.001, ****p < 0.0001

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Molecular Cancer

ISSN: 1476-4598