Fig. 4

CircSMARCC1 acts as a sponge for miR-1322 and miR-1322 reverses the oncogenic effects of circSMARCC1 on proliferation, invasion and migration in PCa cells. A Schematic diagram of circSMARCC1 luciferase reporter vectors carrying wild-type (Wt) or mutant (Mut) miR-1322 binding sites. B Luciferase reporter assay to analyze the effects of 9 candidate miRNAs on the luciferase activity of circSMARCC1. C The RNA pull-down assay performed in DU145 cells using circSMARCC1 and negative control probes. D The relative expression of miR-1322 in PCa cells after transfection of circSMARCC1 was detected by qRT-PCR. E The relative luciferase activities measured in 293 T cells co-transfected with circSMARCC1-Wt or circSMARCC1-Mut and miR-1322 mimics or miR-nc by luciferase reporter assay. F The co-localization of circSMARCC1 and miR-1322 observed using RNA-FISH in DU145 cells (scale bar, 5 μm). The nuclei were stained with DAPI. G-I The viability of PCa cells in miR-1322 rescue experiments was analyzed by colony formation assay, EdU assay (scale bar, 100 μm) and CCK8 assay, respectively. J, K The migration and invasion capacity of PCa cells in the miR-1322 rescue experiment was analyzed by transwell assays (scale bar, 50 μm) and wound healing (scale bar, 100 μm) assays. The data are presented as the mean ± SD, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001