Fig. 3

LncRNA STEAP3-AS1 facilitates migration and invasion of CRC cells. A Representative images of lung (left) and their H&E staining (right) from the tail vein injection model using DLD-1 cells. Scale bar: 100 μm. (sh#1, shSTEAP3-AS1 #1). B The metastatic liver colonization model was generated by inoculating DLD-1 cells into the splenic of BALB/c mice (left), or orthotopically implanting CRC xenograft into the cecum of BALB/c mice (right). Representative H&E images of livers staining are shown. Scale bar: left 100 μm, right 25 μm. (sh#1, shSTEAP3-AS1 #1). C Wound healing assay showing cell migration of DLD-1 (left) and SW480 (right) cells with or without STEAP3-AS1 knockdown. Scale bar: 100 μm. (sh#1, shSTEAP3-AS1 #1; sh#2, shSTEAP3-AS1 #2). D The statistic graph of relative migration distance in (C). (sh#1, shSTEAP3-AS1 #1; sh#2, shSTEAP3-AS1 #2). E-F Migration and invasion of DLD-1 (E) and SW480 cells (F) with or without STEAP3-AS1 knockdown were evaluated using transwell assays. Scale bar: 100 μm. (sh#1, shSTEAP3-AS1 #1; sh#2, shSTEAP3-AS1 #2). G The statistic graph of relative migration cell numbers in (E-F). Left, DLD-1 cells; Right, SW480 cells. (sh#1, shSTEAP3-AS1 #1; sh#2, shSTEAP3-AS1 #2). H EMT markers were detected by western blot in DLD-1 and SW480 cells with or without STEAP3-AS1 knockdown. (sh#1, shSTEAP3-AS1 #1). I-J qPCR analysis of the expression of EMT markers in STEAP3-AS1 knockdown or control DLD-1 (I) and SW480 (J) cells. Data are means ± s.d. and are representative of at least 3 independent experiments. (** P < 0.01 and *** P < 0.001)