Skip to main content
Fig. 6 | Molecular Cancer

Fig. 6

From: Hypoxia-induced lncRNA STEAP3-AS1 activates Wnt/β-catenin signaling to promote colorectal cancer progression by preventing m6A-mediated degradation of STEAP3 mRNA

Fig. 6

STEAP3-AS1 activates wnt/β-catenin signaling to promote CRC progression. A Gene-Concept Network of enriched Wnt signaling pathways based on RNA-sequencing analysis of control and STEAP3-AS1-knockdown DLD-1 cells. B Heatmap of RNA sequencing results from Fig. 6A showing the expression patterns of Wnt signaling pathway-related genes. C Western blotting analysis showing the expression level of cytoplasmic (Cyto) and nuclear (Nuc) β-catenin in DLD-1 and SW480 cells with or without STEAP3-AS1 knockdown. The GSK3β inhibitor CHIR-99021 was used as a positive control. D-E The expression level of cytoplasmic (Cyto) and nuclear (Nuc) β-catenin in control or STEAP3-AS1-overexpressing DLD-1 (D) and HCT116 (E) cells was analyzed by western blotting. F TOP/FOP flash reporter assay in control or STEAP3-AS1-knockdown DLD-1 and SW480 cells. The GSK3β inhibitor CHIR-99021 was used as a positive control. G TOP/FOP flash reporter assay in control or STEAP3-AS1-overexpressing HCT116 (left) and DLD-1 (right) cells. H Relative RNA levels of several Wnt members and the Wnt inhibitor protein DKK1 in control or STEAP3-AS1-knockdown DLD-1 and SW480 cells. The GSK3β inhibitor CHIR-99021 was used as a positive control. Data are means ± s.d. and are representative of at least 3 independent experiments. (** P < 0.01 and *** P < 0.001)

Back to article page

Molecular Cancer

ISSN: 1476-4598