Fig. 4
BAF complex disruption reduces collagen adhesion and invasion in vitro, and causes morphological changes and destruction of stress fibres in neuroblastoma cells. A Adhesion assays of neuroblastoma cells to collagen-coated 96-well plates. Cells were aspirated and rinsed at the indicated time points after seeding. Attached cells were quantified using crystal violet staining and absorbance values were normalized to non-aspirated cells. B Invasion assay with collagen-coated transwells. Neuroblastoma cells (2 × 105 cells/well) were seeded in collagen-coated transwells and let migrate for 16 h. Invaded cells were quantified by crystal violet staining C Representative microscopy pictures invaded cells. D Representative images of neuroblastoma cells stained with phalloidin (F-Actin), anti-tubulin antibody and DAPI by confocal microscopy. E Quantification of tubulin-free phalloidin area per cell of SK-N-BE(2) (upper graph) and SH-SY5Y (lower graph) cell lines. Ten fields per biological replicate n = 3 were analyzed. ** means p < 0.01; *** means p < 0.001