Fig. 7

FUS/circEZH2/KLF5 loop promotes CXCR4-induced EMT program. a Seven ChIP datasets showed that there were KLF5 peaks within 2k bp promotor of CXCR4. b Top, the potential binding sites on the promotor of CXCR4 by KLF5 according to JASPAR. c Dual luciferase assays were conducted in FUS promotor wild type group and E2 mutated group. d According to TCGA GSVA analysis, KLF5 and CXCR4 were positive correlated with EMT markers respectively e Based on STARBASE database, regression correlation rates among KLF5, CXCR4, VIM, CDH1 and CDH2 transcripts were displayed in heatmap. f Western blot was conducted to reveal the change of CXCR4, Vimentin, N-cadherin and E-cadherin after indicated siRNA or Vector. g IF was conducted to find that F-actin were vividly downregulated after knockdown circEZH2 and KLF5 respectively. h and i The CXCR4-rescue colony formation assay and CXCR4-rescue migration Transwell assay were conducted within indicated Vector or drug (Scale bar 100 μm) j IHC were performed in circEZH2 overexpressed group of BCLM nude mice and control group with indicated antibody (Low power field: Scale bar 100 μm; High power field: Scale bar 20µm. ). The data are showed as the mean ± SD and all experiments were repeated at least three times, ns no significant, *P < 0.05 **P < 0.01, ***P < 0.001