Fig. 7

c-Myc promotes circPVT1 generation by recruiting SRSF1 to couple transcription to splicing. A. The expression of circPVT1 and PVT1 in CNE2 and 5-8F cells after overexpression or knockdown of c-Myc was examined using qRT-PCR. Data were represented as mean ± SD; *, p < 0.05; **, p < 0.01; ***, p < 0.001. B. The top 5 splicing factors potentially binding to the exon 2 of PVT1 were listed following analyzed online (RBPsuite: http://www.csbio.sjtu.edu.cn/bioinf/RBPsuite/). The score for SRSF1 is highest (score = 0.9995492). C. The interaction between SRSF1 protein and exon 2 of PVT1 was evaluated in CNE2 and 5-8F cells by RNA immunoprecipitation using anti-SRSF1 antibody, followed by qRT-PCR analysis of the PVT exon 2. Data were represented as mean ± SD. ***, p < 0.001. D. The expresion of circPVT1 and PVT1 in CNE2 and 5-8F cells after overexpression or knockdown of SRSF1 was examined using RT-PCR. Data were represented as mean ± SD; **, p < 0.01; ***, p < 0.001. E. The interaction between SRSF1 and c-Myc proteins was examined in CNE2 and 5-8F cells using immunoprecipitation with anti-c-Myc antibody, followed by western blotting using anti-SRSF1 antibody. F. The expression of SRSF1 in CNE2 and 5-8F cells after overexpression or knockdown of c-Myc was examined using qRT-PCR. Data were represented as mean ± SD; **, p < 0.01; ***, p < 0.001