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Fig. 4 | Molecular Cancer

Fig. 4

From: Long noncoding RNA DLGAP1-AS2 promotes tumorigenesis and metastasis by regulating the Trim21/ELOA/LHPP axis in colorectal cancer

Fig. 4

DLGAP1-AS2 physically interacts with CPSF2, CSTF3 and ELOA in CRC cells. A Proteins retrieved from the DLGAP1-AS2 RNA pull-down assays were analyzed by SDS–PAGE with silver staining. B Immunoblotting analyses of CPSF2, CSTF3 and ELOA in protein retrieved from the DLGAP1-AS2 RNA pull-down assays. C RIP assays were performed to confirm the association of CPSF2, CSTF3 and ELOA with DLGAP1-AS2 using the indicated antibodies. qRT-PCR was used to detect the enrichment of DLGAP1-AS2. D Immunoblotting of CPSF2, CSTF3 and ELOA in samples from the RNA pull-down assays with full-length or truncated DLGAP1-AS2 RNA probes. E-G RIP analyses were performed to measure the enrichment of DLGAP1-AS2 by the full-length or truncated proteins of CPSF2 (E), CSTF3 (F) or ELOA (G)

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Molecular Cancer

ISSN: 1476-4598