Fig. 2

INC280-PFCE NPs uptake efficiency and antitumor effect in vitro. A Representative CLSM images. EBC-1 cells were incubated with rhodamine-labeled INC280-PFCE NPs (10 nM INC280) for 4 h, 12 h and 24 h (red). The nuclei were stained with DAPI (blue). Scale bar is 50 µm. B After incubation for 4 h, 12 h and 24 h, analysis of the mean fluorescence intensity (MFI) of INC280-PFCE NPs in EBC-1 cells. C After incubation with different concentrations of INC280-PFCE NPs (10 nM INC280), viability of EBC-1 cells at 24, 48 and 72 h. D Western blot analysis of MET and its downstream signaling pathway proteins in EBC-1 cells incubated with different treatments for 4 h. The group included Control, PFCE NPs (30 nM PFCE), INC280 (1 nM INC280) and INC280-PFCE NPs (1 nM INC280 and 30 nM PFCE). E After 72 h of treatment with different formulations, detection of EBC-1 cell apoptosis by TUNEL assays. The group included Control, PFCE NPs (300 nM PFCE), INC280 (10 nM INC280) and INC280-PFCE NPs (10 nM INC280 and 300 nM PFCE). Scale bar = 50 µm. F After 72 h of treatment with different formulations, detection of EBC-1 cell apoptosis by flow cytometry. The group included Control, PFCE NPs (300 nM PFCE), INC280 (10 nM INC280) and INC280-PFCE NPs (10 nM INC280 and 300 nM PFCE). G Quantification of cell apoptosis analyzed by flow cytometry. H After 72 h of treatment with different formulations, cell cycle analyzed by flow cytometry. The group included Control, PFCE NPs (300 nM PFCE), INC280 (10 nM INC280) and INC280-PFCE NPs (10 nM INC280 and 300 nM PFCE). I Quantitative analysis of EBC-1 cells in G1, S and G2 phases. Data are shown as mean ± standard deviation (n = 3; * P < 0.05, **** P < 0.0001)