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Fig. 1 | Molecular Cancer

Fig. 1

From: circCYP24A1 facilitates esophageal squamous cell carcinoma progression through binding PKM2 to regulate NF-κB-induced CCL5 secretion

Fig. 1

Characteristics of circCYP24A1 in ESCC. a A volcano plot shows 10,633 differentially expressed circRNAs in five paired samples of ESCC by RNA-Seq. Cut off is Log 2 (Fold Change) > 1.0 or <  − 1.0, P value < 0.05. b Upper panel: The genomic structure and backsplice junction site of circCYP24A1 was analyzed by circBase database and Sanger sequencing. Lower panel: The expression of circCYP24A1 in KYSE30 and KYSE150 cells was detected by RT-PCR. c The expression of circCYP24A1 in ESCC cells was detected by qRT-PCR. d RNA abundance of circCYP24A1 and CYP24A1 treated with Actinomycin D (2 μg/ml) was detected by qRT-PCR. ***P < 0.001. f Resistance of circCYP24A1 and CYP24A1 to digestion with RNase R exonuclease was detected by qRT-PCR. **P < 0.01, ***P < 0.001. g RNA FISH assay displayed that circCYP24A1 mainly localized in cytoplasm in ESCC cells

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Molecular Cancer

ISSN: 1476-4598