Fig. 6
In vivo effects of over-expression of the soluble and the intracellular OPN in OPN-/-Faslpr/lpr DLBCL cells. A. Flow cytometry analysis showing the tumour take (% of CD19 + GFP +) in spleen and liver of 5 Gy irradiated BALB/c mice injected with OPL239 IRES-Green, OPL239 Spp1-IRES-Green and OPL239 iOPN-IRES-Green. Four mice per group were used in the experiment (*, p < 0.05; ****, p < 0.0001; Ordinary one way ANOVA). B. Flow cytometry analysis showing the percentage of lymphoid and myeloid populations in the spleen of mice injected with OPL239 cell variants [(T conv: T conventional cells; T reg: T regulatory cells) (*, p < 0.05, **, p < 0.001; ****, p < 0.0001; Ordinary one way ANOVA)]. An irradiated B/c mouse was used as control