Fig. 2

miR-1 modulates growth and migration of SCLC cells. A miR-1 expression in DOX-On-miR-1 SBC5 cells induced with DOX for 24–48 h assessed by TaqMan-based qPCR assay. RNU6B was used to normalize the gene expression. Representative images from sphere formation assay from, (B) SBC5 no miR-1(-DOX-Off) and + miR-1 (+ DOX-On), (C) SBC3 and SBC3-miR-1Zip cells. Representative images for colony formation assay from, (D) SBC5 no miR-1(-DOX-Off) and + miR-1 (+ DOX-On) cells, the right panel showed the quantification of number of colonies from n = 3 biological replicates, (E) SBC3 and SBC3-miR-1Zip cells, the lower panel showed the quantification of the number of colonies from n = 3 biological replicates. Quantification of percent wound closure area from real-time monitoring for cell migration assay of, (F) SBC5 no miR-1(-DOX-Off) and + miR-1 (+ DOX-On) cells, (G) SBC3 and SBC3-miR-1Zip cells. H FACS analysis of the apoptotic potential of miR-1 in SBC5/NCI-H69 cells under no miR-1(-DOX-Off) and + miR-1 (+ DOX-On) conditions as monitored for 24–72 h. The cells were induced for miR-1 expression for the indicated period (24-72 h) and stained with annexin-V/PI to analyze apoptosis induction by flow cytometry. Quantification of apoptosis was presented in the right panels (from n = 3 biological replicates). Statistical significance was considered using a two-tailed Student’s t-test, except in H, where ordinary one-way ANOVA was used. *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001