Fig. 4
From: Targeting SOST using a small-molecule compound retards breast cancer bone metastasis
Screening and identification of S6, a small-molecule targeting SOST. A A scheme for computational screening of inhibitors that block STAT3 binding to SOST. B Protein–protein interface of SOST and STAT3. C Docking of S6 with the SOST pocket. D Structure of compound 6, named S6. E Multi-concentration gradient detection of S6 (KD = 3.992E-04, R2 = 0.9594). F Inhibitory effect of S6 on the viability of SCP2 cells. G Total energy of SOST-S6 complex. H Overall structure and close-up views of the SOST-S6-STAT3 complex. I A scheme for S6 blocking SOST-STAT3 binding. J Co-immunoprecipitation reveals that S6 inhibits the SOST-STAT3 binding in SCP2 cells